Effects of Eluting Volumes to Isolation Efficiencies in Manual Synthesis of Ga-68 Labelled FAPI-04

Abstract Among different FAPIs (fibroblast activation protein inhibitors) developed for PET imaging, 68 Ga-FAPI-04 has demonstrated the most impressive properties with low nanomolar affinity to FAP, near-complete internalization of radioactivity bound and rapid blood clearance. The application been extended 28 kinds clinical cancer detection. manual synthesis is like other Ga-labeling peptides, such as PSMA-11 DOTATATE. However, because radiochemical conversion (RCC) about 90%, it required conduct a purification isolation process meet standard application. purpose this work characterize increase efficiency (IE) by increasing volume eluting liquid applied C18 columns sterile filters. We designed an experiment measured residual activity distribution on both filters volumes. characterized change residuals efficiencies volumes in isolation. As result, was found that there were more leftovers than columns. By from 6 mL 12 mL, we average IE being improved 62.4% 87.4%, which greatly beneficial applications. In addition, fluctuation might come radiolabeling operators or materials used experiment, also obviously decreased 11.3% 4.5%. This method proven be efficient production Ga-FAPI-04.